pBABE-neo载体质粒图谱、序列、价格、抗性、测序引物、大小等基本...

来自 : www.biofeng.com/plus/vi...php? 发布时间：2021-03-25

/translation= MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF* gene 1773..2561 /label= NeoR/KanR /gene= NeoR/KanR /translation= MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF* misc_feature 2611..3204 /label= 3MoMuLV_LTR /translation= MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF* misc_feature 2653..3204 /label= 5_LTR2 /translation= MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF* gene complement(4342..5202) /label= Ampicillin /gene= Ampicillin /translation= MTEYKPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFR LSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGE VPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRL GVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF* CDS complement(4342..5202) /label= ORF frame 3 /translation= MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW* promoter complement(5244..5272) /label= AmpR_promoter /translation= MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW* ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga 1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc 1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 1741 aaaagcttac catgaccgag tacaagccca tgattgaaca agatggattg cacgcaggtt 1801 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 1861 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 1921 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 1981 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 2041 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 2101 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 2161 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 2221 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 2281 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 2341 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 2401 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 2461 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 2521 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagatcga taaaataaaa 2581 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa 2641 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa 2701 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt 2761 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc 2821 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc 2881 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc 2941 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt 3001 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg 3061 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc 3121 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact 3181 acccgtcagc gggggtcttt catttccgac ttgtggtctc gctgccttgg gagggtctcc 3241 tctgagtgat tgactacccg tcagcggggg tcttcacatg cagcatgtat caaaattaat 3301 ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat gaatcggcca 3361 acgcgcgggg agaggcggtt tgcgtattgg cgctcttccg cttcctcgct cactgactcg 3421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 3541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 3601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 3661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 3721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 3781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 3841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 3901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 3961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 4021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 4081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 4141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 4201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 4261 acctagatcc ttttaaatta aaaatgaagt ttgcggccgc aaatcaatct aaagtatata 4321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 4381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 4441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 4501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 4561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 4621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 4681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 4741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 4801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 4861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 4921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 4981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 5041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 5101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 5161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 5221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 5281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac